Journal: The journals of gerontology. Series A, Biological sciences and medical sciences

Article Title: Resveratrol improves insulin resistance hyperglycemia and hepatosteatosis but not hypertriglyceridemia, inflammation, and life span in a mouse model for Werner syndrome.

doi: 10.1093/gerona/glq184

Figure Lengend Snippet: Figure 7. Resveratrol effect on energy production (ATP) and AMPKa activity of 9-month-old WrnDhel/Dhel mice in liver tissues. (A) Liver GSH levels in 9-month- old wild-type (WT), WrnDhel/Dhel, and resveratrol-treated WrnDhel/Dhel mice. Measurements were taken from four mice of each group. (B) Liver ATP levels in 9-month- old WT, WrnDhel/Dhel, and resveratrol-treated WrnDhel/Dhel mice. (C) Example of Western blots showing levels of AMPKa phosphorylation at its threonine 172 (p-AMPKa) in liver tissues of all three cohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated AMPKa over total AMPKa detected in the liver of mice. (D) Example of Western blots showing levels of acetyl-CoA carboxylase phosphorylation at its serine 79 (p-ACC) in liver tissues of all three cohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated ACC over total ACC detected in the liver of mice. (E) Example of Western blots showing levels of hormone-sensitive lipase (HSL) phosphorylation at its serine 565 (p-HSL) in liver tissues of all three c ohorts. The graph represents scanning analyses of Western blots expressed as the average signal from the phosphorylated HSL over total HSL detected in the liver of mice. Measurements were taken from four to six males in each group. Bars in the histograms represent the SEM. (* = unpaired t test; p < .05 when compared with WrnDhel/Dhel mice). (F) Example of Western blots showing levels of fatty acid synthase (FASN) in liver tissues of all three cohorts. The gene product HNRPK was used as loading control. The graph on the right represents scanning analyses of Western blots expressed as the average signal from the FASN signal over HNRPK signal detected in the liver of mice. Measurements were taken from three males in each group. Bars in the histograms represent the SEM. (* = unpaired t test; p < .02 when compared with WrnDhel/Dhel mice).

Article Snippet: The rabbit monoclonal antibodies against AMPKa (23A3), against AMPKa phosphorylated on threonine 172 (40H9), against acetyl-CoA carboxylase (C83B10), against phosphorylated acetyl-CoA carboxylase on Serine 79, against the hormone-sensitive lipase (HSL), and against the phosphorylated HSL on Serine 565 were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: Activity Assay, Western Blot, Phospho-proteomics, Control

Journal: International journal of molecular medicine

Article Title: AMP-activated protein kinase regulates thyroid hormone-stimulated osteocalcin synthesis in osteoblasts.

doi: 10.3892/ijmm.2013.1349

Figure Lengend Snippet: Figure 3. Effect of compound C on the T3-induced phosphorylation of acetyl- CoA carboxylase in MC3T3-E1 cells. The cultured cells were pretreated with various doses of compound C for 60 min, and then stimulated by 10 nM T3 for 60 min. The extracts of cells were subjected to SDS-PAGE with subsequent western blot analysis with antibodies against phospho-specific acetyl-CoA carboxylase or GAPDH.

Article Snippet: �h���h�-�����f�� AM��α (Thr-172) antibodies, phosphospecific AMPKα (Ser-485) antibodies, AMPKα antibodies, �h���h�-�����f�� AM��β (Ser-108) antibodies, phospho�����f�� AM��β (Ser-182) antibodies, AMPKβ antibodies and phospho-specific acetyl-CoA carboxylase antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Phospho-proteomics, Cell Culture, SDS Page, Western Blot

Journal: International journal of molecular medicine

Article Title: AMP-activated protein kinase regulates thyroid hormone-stimulated osteocalcin synthesis in osteoblasts.

doi: 10.3892/ijmm.2013.1349

Figure Lengend Snippet: Figure 2. Effect of T3 on the phosphorylation of acetyl-CoA carboxylase in MC3T3-E1 cells. The cultured cells were stimulated by 10 nM T3 for the indicated periods. The extracts of cells were subjected to SDS-PAGE with subsequent western blot analysis with antibodies against phospho-specific acetyl-CoA carboxylase or GAPDH.

Article Snippet: �h���h�-�����f�� AM��α (Thr-172) antibodies, phosphospecific AMPKα (Ser-485) antibodies, AMPKα antibodies, �h���h�-�����f�� AM��β (Ser-108) antibodies, phospho�����f�� AM��β (Ser-182) antibodies, AMPKβ antibodies and phospho-specific acetyl-CoA carboxylase antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Phospho-proteomics, Cell Culture, SDS Page, Western Blot

Journal: Environment international

Article Title: Cresyl diphenyl phosphate (a novel organophosphate ester) induces hepatic steatosis by directly binding to liver X receptor α: From molecule action to risk assessment.

doi: 10.1016/j.envint.2024.109168

Figure Lengend Snippet: Fig. 6. Lipogenesis effects of CDP on mouse liver. a-h 6-week-old mice were intragastric administrated with different dose of CDP (0.1, 1, 10 mg/kg/day) for 12 weeks with corn oil as control treatment (n = 7 per group). (a) H&E and Oil red O staining of liver sections. (b-f) Relative Lxrα, Srebp1c, Acc1, Fasn, and Scd mRNA expression levels in mouse liver. The relative mRNA level was normalized to control group (mice treated with corn oil). *P < 0.05, compared with control. (g) Protein expression of LXRα, SREBP1c, ACC1, FASN, and SCD in mouse liver. (h) Quantitative analysis of protein expression.

Article Snippet: The following antibodies were used in the study: anti-LXRα antibody (Proteintech, catalogue no. 14351-1-AP, diluted 1:5000), anti-SREBP1c antibody (Zen bio, catalogue no. 347061, diluted 1:1000), anti-FASN antibody (Zen bio, catalogue no. 200194, diluted 1:1000), anti-ACC1 antibody (Proteintech, catalogue no. 21923-1-AP, diluted 1:5000), anti-SCD antibody (Zen bio, catalogue no. R25675, diluted 1:1000).

Techniques: Control, Staining, Expressing